2ZTG
Crystal structure of Archaeoglobus fulgidus alanyl-tRNA synthetase lacking the C-terminal dimerization domain in complex with Ala-SA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-06-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97888, 0.97936 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.391, 49.082, 98.044 |
| Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
| Resolution | 46.740 - 2.200 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.509 |
| Number of reflections | 37240 | |
| <I/σ(I)> | 24 | 2.2 |
| Completeness [%] | 99.4 | 95.2 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 18% PEG-MME 2000, 5mM MgCl2, 0.25mM AlaSA, 100mM Bis-Tris, 0.1mM zinc acetate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
