2ZTE
MtRuvA Form IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 84.340, 84.340, 100.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.637 - 3.200 |
| R-factor | 0.2297 |
| Rwork | 0.230 |
| R-free | 0.30690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.138 | 0.462 |
| Number of reflections | 3241 | |
| <I/σ(I)> | 12.9 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | Crystallized in the presence of excess Holliday Junction (1:1.5) with 0.1M sodium succinate, pH5.0, 1.7M ammonium sulfate as the precipitant, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
