2ZTB
Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3403, 1.3408, 1.3460 |
| Spacegroup name | P 65 |
| Unit cell lengths | 136.829, 136.829, 119.268 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.740 - 2.380 |
| R-factor | 0.19377 |
| Rwork | 0.191 |
| R-free | 0.22212 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.337 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.067 | 0.451 |
| Number of reflections | 50770 | |
| <I/σ(I)> | 26.2 | 4.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 11 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 16% (v/v) ethylene glycol, 8% (w/v) PEG 3350, 1mM TCEP, 50mM HEPES-NaOH, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
