2ZSC
Tamavidin2, Novel Avidin-like Biotin-Binding Proteins from an Edible Mushroom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.382, 80.017, 55.733 |
| Unit cell angles | 90.00, 132.32, 90.00 |
Refinement procedure
| Resolution | 18.710 - 1.300 |
| R-factor | 0.20136 |
| Rwork | 0.199 |
| R-free | 0.23758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1slg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.118 | 0.470 |
| Number of reflections | 61099 | |
| <I/σ(I)> | 2 | 1 |
| Completeness [%] | 97.4 | 100 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.4 | 25% PEG 3350, 0.2M MgCl2, 0.1M Bis-Tris pH 5.5, 0.1M NaCl, 0.025M MES pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.4K |
