2ZR8

Crystal Structure of Modified Serine Racemase complexed with Serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-19
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	59.540, 72.880, 64.810
Unit cell angles	90.00, 101.73, 90.00

Refinement procedure

Resolution	19.570 - 2.200
R-factor	0.171
Rwork	0.171
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zpu
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	13720
Completeness [%]	99.2	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	21% PEG 4000, 0.2M Magnesium Acetate, 10% MPD, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K