2ZR7
Msreca native form II'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 296 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 103.619, 103.619, 74.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.910 - 3.600 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.142 | 0.496 |
| Number of reflections | 5133 | |
| <I/σ(I)> | 7.1 | 1.6 |
| Completeness [%] | 95.8 | 97.6 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 296 | 80mM citrate/phosphate buffer (pH 6.9), 80mM NaCl, 40mM ammonium acetate, 20mM sodium citrate, 6% poly-ethylene glycol 3350, 30% poly-ethylene glycol 3350, 200mM ammonium acetate in sodium citrate buffer (PH 5.8) , VAPOR DIFFUSION, HANGING DROP, temperature 296K |
