2ZQZ
R-state structure of allosteric L-lactate dehydrogenase from Lactobacillus casei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.382, 82.893, 179.771 |
| Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
| Resolution | 34.100 - 2.500 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.510 |
| Number of reflections | 168255 | |
| <I/σ(I)> | 6.1 | 1.5 |
| Completeness [%] | 99.9 | 93.3 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 1.6M ammonium sulfate, 0.1M sodium citrate, 0.2M potassium sodium tartrate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
