2ZQY
T-state structure of allosteric L-lactate dehydrogenase from Lactobacillus casei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.337, 143.984, 144.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.820 - 2.600 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 43900 | |
| <I/σ(I)> | 7.5 | 1.9 |
| Completeness [%] | 93.2 | 86 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 20% PEG 6000, 0.2M Mg(NO3)2, 10mM oxamate, 1mM NADH, 5mM FBP, 10mM MnSO4 , pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
