2ZQR

Crystal structure of AUH without RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-27
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	79.208, 132.429, 80.043
Unit cell angles	90.00, 108.10, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.197
Rwork	0.197
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzd
RMSD bond angle	1.190
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.089	0.340
Number of reflections	53373
<I/σ(I)>	8.1	2.19
Completeness [%]	98.4	95.9
Redundancy	2.7	2.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	DROP: 50MM SODIUM CACODYLATE, 9% PEG8000, 100MM CALCIUM ACETATE, 100MM SODIUM ACETATE, 10MM IMIDAZOLE, RESERVOIR: 100MM SODIUM CACODYLATE, 18% PEG8000, 200MM CALCIUM ACETATE, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K