2ZQK
Crystal structure of intimin-Tir68 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.680, 42.310, 182.080 |
| Unit cell angles | 90.00, 101.13, 90.00 |
Refinement procedure
| Resolution | 34.100 - 2.800 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f02 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.376 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 | 0.184 |
| Number of reflections | 20419 | |
| <I/σ(I)> | 25.4 | 6.8 |
| Completeness [%] | 93.6 | 65.5 |
| Redundancy | 6.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 0.1M HEPES sodium, 2% v/v PEG 400, 2.0M Ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
