2ZPT
Crystal structure of mouse sulfotransferase SULT1D1 complex with PAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.819, 67.600, 42.696 |
| Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
| Resolution | 20.560 - 1.150 |
| R-factor | 0.14856 |
| Rwork | 0.148 |
| R-free | 0.16018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.044 | 0.356 |
| Number of reflections | 142203 | |
| <I/σ(I)> | 22.3 | 2.1 |
| Completeness [%] | 94.3 | 69.2 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 16% PEG 10000, 10mM dithiothreitol, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
