2ZPN

The crystal structure of Saccharomyces cerevisiae Atg8- Atg19(412-415) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-10-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	44.150, 104.450, 113.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.100 - 2.700
R-factor	0.242
Rwork	0.242
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.100	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.073	0.260
Number of reflections	14927
<I/σ(I)>	10	5.12
Completeness [%]	99.1	98.4
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	2.0M ammonium sulfate, 0.1M sodium citrate, pH4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K