2ZPL
Crystal structure analysis of PDZ domain A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 |
| Unit cell lengths | 54.756, 54.756, 75.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.550 - 1.700 |
| R-factor | 0.15075 |
| Rwork | 0.150 |
| R-free | 0.17550 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | and (SCALA) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.550 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 | 0.376 |
| Number of reflections | 27607 | |
| <I/σ(I)> | 35.1 | 4.2 |
| Completeness [%] | 99.0 | 93.9 |
| Redundancy | 10.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 17% PEG2000mme, 85mM Tris-HCl, 8.5mM NiCl2, VAPOR DIFFUSION, temperature 293K |
