2ZPK
Crystal structure of P20.1 Fab fragment in complex with its antigen peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-12 |
| Detector | DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.045, 65.271, 85.030 |
| Unit cell angles | 99.93, 93.50, 96.46 |
Refinement procedure
| Resolution | 46.780 - 1.800 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nc4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.470 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.234 |
| Number of reflections | 76301 | |
| <I/σ(I)> | 10.3 | 3.2 |
| Completeness [%] | 97.8 | 95.3 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20-23% (wt/vol) PEG 3000, 0.1M Sodium acetate (pH4.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
