2ZPC

Crystal structure of the R43L mutant of LolA in the closed form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	90
Detector technology	CCD
Collection date	2004-03-09
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	60.982, 60.982, 78.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.350
Rwork	0.241
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ua8
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	7164
<I/σ(I)>	46.8	3.2
Completeness [%]	96.7	84.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	15% PEG 3350, 50mM MES (pH 6.5), 20% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K