2ZP9

The Nature of the TRAP:Anti-TRAP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 6
Unit cell lengths	197.134, 197.135, 56.658
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.30461
Rwork	0.303
R-free	0.32480
Structure solution method	PHASER
Starting model (for MR)	2bx9 1qaw
RMSD bond length	0.008
RMSD bond angle	1.124
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.056	0.195
Number of reflections	19198
<I/σ(I)>		2.2
Completeness [%]	90.7	75.9
Redundancy	3.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M succinate pH 7.0, 13-15% PEG 10000, 2% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 293K