2ZP6
Crystal structure of Bovine Insulin (Hexameric form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.465, 82.465, 33.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.750 - 2.560 |
| R-factor | 0.20007 |
| Rwork | 0.198 |
| R-free | 0.21956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a3g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.227 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.750 | 2.640 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 2479 | |
| <I/σ(I)> | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15% acetone, 0.6M Ammonium formate (metal basis), 0.6M sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
