2ZOZ

Crystal structure of the ethidium-bound form of the multi-drug binding transcriptional repressor CgmR

Replaces:  2DH0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-04
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.745, 92.226, 105.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 1.950
R-factor	0.207
Rwork	0.207
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v7b
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.076	0.245
Number of reflections	45773
<I/σ(I)>	19.5
Completeness [%]	98.4	96.2
Redundancy	7.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.6M LiSO4, 10mM MgCl2, 0.1M Hepes, 1mM ethidium bromide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K