2ZOY

The multi-drug binding transcriptional repressor CgmR (CGL2612 protein) from C.glutamicum

Replaces:  1V7B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	59.754, 67.051, 101.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.2
Rwork	0.200
R-free	0.23200
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.066	0.298
Number of reflections	32643
<I/σ(I)>	4.5
Completeness [%]	99.7	99.7
Redundancy	4.3	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	21% PEG 400, 0.16M Magnesium Chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K