2ZOU
Crystal structure of human F-spondin reeler domain (fragment 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-10-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.253, 50.518, 63.171 |
| Unit cell angles | 90.00, 100.37, 90.00 |
Refinement procedure
| Resolution | 62.140 - 1.450 |
| R-factor | 0.20306 |
| Rwork | 0.202 |
| R-free | 0.22248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zot |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 48350 | |
| <I/σ(I)> | 15.1 | 3.3 |
| Completeness [%] | 94.5 | 93.6 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M bis-tris (pH 5.5), 0.4M ammonium acetate, 25% polyethylene glycol(PEG) 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
