2ZOS
Crystal structure of mannosyl-3-phosphoglycerate phosphatase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9793, 0.9641 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.487, 68.607, 67.567 |
| Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| Rwork | 0.213 |
| R-free | 0.23420 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.224 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 61540 | |
| <I/σ(I)> | 14.6 | 2.3 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 16% PEG 3350, 0.1M MES, 0.2M ammonium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
