2ZOP
X-ray crystal structure of a CRISPR-associated Cmr5 family protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9790 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.122, 114.801, 122.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.620 - 2.100 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.064 | 0.314 |
| Number of reflections | 45600 | |
| <I/σ(I)> | 51.1 | 9.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1M sodium acetate, 1.7M ammonium sulfate, 0.03M DTT, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
