2ZON

Crystal structure of electron transfer complex of nitrite reductase with cytochrome c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-30
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	63.345, 103.942, 163.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.16548
Rwork	0.162
R-free	0.19542
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oe1 1kx7
RMSD bond length	0.011
RMSD bond angle	1.496
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.800	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	119863
Completeness [%]	98.8	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	277	PEG 3350, Magnesium acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K