2ZOG
Crystal structure of mouse carnosinase CN2 complexed with ZN and bestatin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 1.2827, 1.2834, 1.2573 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.417, 199.779, 55.495 |
| Unit cell angles | 90.00, 118.52, 90.00 |
Refinement procedure
| Resolution | 39.340 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.584 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.038 | 0.342 |
| Number of reflections | 109231 | |
| Completeness [%] | 96.1 | 74.4 |
| Redundancy | 5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 20% PEG 3350, 20% GLYCEROL, 0.2M HF, 25MH TRIS-HCL, 50MM NACL, 0.2MM MNCL2, 1MM DTT, 30MM BESTATIN, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
