2ZNM
Oxidoreductase NmDsbA3 from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.469, 88.531, 84.295 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.300 |
| R-factor | 0.20927 |
| Rwork | 0.206 |
| R-free | 0.26338 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 34110 | |
| <I/σ(I)> | 43.3 | 5 |
| Completeness [%] | 94.0 | 65 |
| Redundancy | 5.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 20% PEG4000, 0.1M Tris, pH8.0, 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
