2ZNC
MURINE CARBONIC ANHYDRASE IV
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.500, 85.200, 121.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1znc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.083 |
| Total number of observations | 26921 * |
| Number of reflections | 6750 * |
| Completeness [%] | 94.8 |
| Redundancy | 3.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.6 * | 4 * | pH 7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 15 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 20 (%) | |
| 4 | 1 | reservoir | Tris-sulfate | 100 (mM) |
