2ZNB
METALLO-BETA-LACTAMASE (CADMIUM-BOUND FORM)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-05-30 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.086, 78.086, 139.746 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.150 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.25900 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1ZNB WITHOUT METALS OR SOLVENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.700 * |
| Data reduction software | MADNES |
| Data scaling software | PROCOR |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.330 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 * | |
| Number of reflections | 26096 | |
| Completeness [%] | 87.4 | 74.1 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | Concha, N.O., (1996) Structure (London), 4, 823. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 55 (mM) | |
| 3 | 1 | drop | 0.01 (mM) | ||
| 4 | 1 | drop | PEG2000 | 26-28 (%) | |
| 5 | 1 | reservoir | PEG2000 | 26-28 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
