2ZMN
Crystal Structure of basic winged bean lectin in complex with Gal-alpha- 1,6 Glc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 158.061, 91.758, 73.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.23700 |
| Starting model (for MR) | 1wbl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.124 | 0.489 |
| Number of reflections | 23768 | |
| <I/σ(I)> | 7.4 | 2.8 |
| Completeness [%] | 97.6 | 98.2 |
| Redundancy | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 300 | 6-7% PEG 4000, 5% isopropenol, 20mM PBS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
