2ZML
Crystal structure of basic winged bean lectin in complex with Gal-ALPHA 1,4 Gal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 157.928, 91.524, 73.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.550 - 2.650 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24700 |
Starting model (for MR) | 1wbl |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.119 | 0.467 |
Number of reflections | 30795 | |
<I/σ(I)> | 15.8 | 2.4 |
Completeness [%] | 97.0 | 99 |
Redundancy | 3.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |