2ZMC
Crystal structure of human mitotic checkpoint kinase Mps1 catalytic domain apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9200 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.430, 103.940, 110.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.400 - 3.140 |
| R-factor | 0.22603 |
| Rwork | 0.223 |
| R-free | 0.28902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1phk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.440 | 3.310 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.123 | 0.636 |
| Number of reflections | 7029 | |
| <I/σ(I)> | 13.1 | 2.8 |
| Completeness [%] | 96.4 | 96.4 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG300, HEPES, pH6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
