2ZM6
Crystal structure of the Thermus thermophilus 30S ribosomal subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-03-22 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 411.500, 411.500, 172.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 184.030 - 3.300 |
| Rwork | 0.292 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e5l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.146 | 0.673 |
| Number of reflections | 235783 | |
| <I/σ(I)> | 5.72 | 1.9 |
| Completeness [%] | 97.7 | 97.2 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 10mM HEPES-KOH, 10mM MgCl2, 60mM NH4Cl, 6mM beta-mercaptoethanol, 11-12% MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES-KOH | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | NH4Cl | ||
| 4 | 1 | 1 | beta-mercaptoethanol | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | HEPES-KOH | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | NH4Cl | ||
| 9 | 1 | 2 | MPD |
