2ZLX
Horse methemoglobin high salt, pH 7.0 (66% relative humidity)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.445, 63.398, 102.590 |
| Unit cell angles | 90.00, 120.98, 90.00 |
Refinement procedure
| Resolution | 19.540 - 2.800 |
| R-factor | 0.26157 |
| Rwork | 0.260 |
| R-free | 0.29349 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mhb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.448 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.173 | 0.344 |
| Number of reflections | 11560 | |
| <I/σ(I)> | 3.2 | 2.1 |
| Completeness [%] | 77.7 | 82.2 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7 | 298 | 2.6M ammonium sulfate, 0.01M ammonium phosphate, pH 7.0, LIQUID DIFFUSION, temperature 298.0K |
