2ZLW
Horse methemoglobin high salt, pH 7.0 (75% relative humidity)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.272, 63.265, 108.149 |
| Unit cell angles | 90.00, 111.28, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.900 |
| R-factor | 0.27463 |
| Rwork | 0.274 |
| R-free | 0.29363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mhb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.504 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.185 | 0.378 |
| Number of reflections | 14192 | |
| <I/σ(I)> | 4.6 | 2.7 |
| Completeness [%] | 92.9 | 94.5 |
| Redundancy | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7 | 298 | 2.6M ammonium sulfate, 0.01M ammonium phosphate, pH 7.0, LIQUID DIFFUSION, temperature 298.0K |
