2ZLV
Horse methemoglobin high salt, pH 7.0 (79% relative humidity)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.096, 62.942, 53.156 |
| Unit cell angles | 90.00, 115.27, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.000 |
| R-factor | 0.20014 |
| Rwork | 0.198 |
| R-free | 0.22988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mhb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.341 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.126 | 0.393 |
| Number of reflections | 18738 | |
| <I/σ(I)> | 7.7 | 2.8 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7 | 298 | 2.6M ammonium sulfate, 0.01M ammonium phosphate, pH 7.0, LIQUID DIFFUSION, temperature 298.0K |
