2ZLT
Horse methemoglobin high salt, pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.650, 63.336, 54.957 |
| Unit cell angles | 90.00, 110.84, 90.00 |
Refinement procedure
| Resolution | 18.320 - 1.900 |
| R-factor | 0.19197 |
| Rwork | 0.190 |
| R-free | 0.22592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mhb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.242 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.320 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.136 | 0.528 |
| Number of reflections | 26933 | |
| <I/σ(I)> | 8.7 | 3.3 |
| Completeness [%] | 97.6 | 99.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7 | 298 | 2.6M ammonium sulfate, 0.01M ammonium phosphate, pH 7.0, LIQUID DIFFUSION, temperature 298.0K |
