2ZLF
The Structural Basis for Peptidomimetic Inhibition of Eukaryotic Ribonucleotide Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90020 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.948, 116.999, 63.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 2.590 |
| R-factor | 0.19554 |
| Rwork | 0.192 |
| R-free | 0.25143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cvx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.459 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.656 |
| High resolution limit [Å] | 2.590 | 2.589 |
| Number of reflections | 24929 | |
| <I/σ(I)> | 21.5 | 2.1 |
| Completeness [%] | 96.5 | 82.4 |
| Redundancy | 6.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM Hepes, 20-25% PEG 3350, 0.2M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
