2ZL8
Crystal structure of copper amine oxidase from Arthrobacter globiformis: Substrate Schiff-base intermediate formed with ethylamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-10 |
| Detector | MAC Science DIP-2040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.759, 63.519, 184.074 |
| Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
| Resolution | 26.400 - 1.730 |
| Rwork | 0.191 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.370 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.076 | 0.309 |
| Number of reflections | 172009 | |
| Completeness [%] | 97.6 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 1.05M potassium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
