2ZL7
Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.069, 83.069, 164.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.070 - 1.350 |
| R-factor | 0.17162 |
| Rwork | 0.171 |
| R-free | 0.18804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.061 | 0.419 |
| Number of reflections | 143419 | |
| <I/σ(I)> | 11.2 | 2.6 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 4.66 | 3.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 50mM Sodium acetate buffer(pH 4.8), 0.2M Ammonium Nitrate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
