2ZL5
Atomic resolution structural characterization of recognition of histo-blood group antigen by Norwalk virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.517, 83.517, 163.368 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.100 - 1.470 |
| R-factor | 0.18178 |
| Rwork | 0.181 |
| R-free | 0.19992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.386 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.760 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.066 | 0.498 |
| Number of reflections | 111763 | |
| <I/σ(I)> | 10.1 | 2.7 |
| Completeness [%] | 99.1 | 98.1 |
| Redundancy | 5.99 | 6.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 50mM Sodium acetate buffer(pH 4.8), 0.2M Ammonium Nitrate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
