2ZL2

Crystal structure of H.pylori ClpP in complex with the peptide NVLGFTQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	92.741, 166.425, 187.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.500
R-factor	0.23
Rwork	0.230
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zl0
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Number of reflections	100644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	32% PEG 400, 0.1M HEPES, 0.35M magnesium chloride, pH 7.5, Microbatch, temperature 298K