2ZL0

Crystal structure of H.pylori ClpP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-01
Detector	BRUKER PROTEUM 300
Wavelength(s)	1.12714
Spacegroup name	P 21 21 21
Unit cell lengths	92.563, 166.225, 187.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 2.600
R-factor	0.231
Rwork	0.231
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tyf
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Number of reflections	82258

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	287	32% PEG400, 0.1M HEPES, 0.35M magnesium chloride, pH 7.5, Microbatch, temperature 287K