2ZKT
Structure of PH0037 protein from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-21 |
| Detector | RIGAKU |
| Wavelength(s) | 1.282247, 1.282786, 1.33 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 155.900, 155.900, 230.857 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.27749 |
| Rwork | 0.277 |
| R-free | 0.28556 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.062 |
| Number of reflections | 39476 |
| <I/σ(I)> | 10.2 |
| Completeness [%] | 99.9 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.6 | 295 | 18% PEG 20K, 0.1M Tris_Hcl, 0.5M licl2, pH 6.6, VAPOR DIFFUSION, temperature 295K |
