2ZKL

Crystal Structure of capsular polysaccharide assembling protein CapF from staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-22
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	119.636, 119.636, 129.454
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.610
R-factor	0.238
Rwork	0.238
R-free	0.27300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.054	0.503
Number of reflections	32990
Completeness [%]	99.7	100
Redundancy	11	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	300mM sodium chloride, 100mM ammonium sulfate, 100mM MES, 3.9M HCOONa-HCl(pH6.3), 30%(w/v) Glycerol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	300mM sodium chloride, 100mM ammonium sulfate, 100mM MES, 3.9M HCOONa-HCl(pH6.3), 30%(w/v) Glycerol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K