2ZJT

Crystal structure of dna gyrase B' domain sheds lights on the mechanism for T-segment navigation

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	93
Detector technology	CCD
Collection date	2006-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97947, 1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.831, 52.763, 192.579
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.030 - 2.800
R-factor	0.244
Rwork	0.244
R-free	0.28000
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.700	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.049	0.348
Number of reflections	13423
<I/σ(I)>	9.5	2.2
Completeness [%]	96.3	88.4
Redundancy	5.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	15% PEG 3350, 50mM sodium acetate, 0.2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5	277	15% PEG 3350, 50mM sodium acetate, 0.2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K