2ZJA
Archaeal DNA helicase Hjm complexed with AMPPCP in form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.145, 81.627, 86.635 |
| Unit cell angles | 90.00, 112.83, 90.00 |
Refinement procedure
| Resolution | 38.250 - 2.700 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zj8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 | 0.408 |
| Number of reflections | 23998 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.7 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 80mM Tris, 160mM CaCl2, 11% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
