2ZJ8
Archaeal DNA helicase Hjm apo state in form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-25 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.346, 81.155, 85.192 |
| Unit cell angles | 90.00, 111.92, 90.00 |
Refinement procedure
| Resolution | 45.990 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zj2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.450 | |
| Number of reflections | 51591 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 97.6 | 91.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.6M ammonium sulfate, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
