2ZJ7
Crystal structure of D157A mutant of Pseudomonas sp. MIS38 lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-14 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.634, 84.627, 87.521 |
| Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.210 |
| R-factor | 0.16806 |
| Rwork | 0.164 |
| R-free | 0.23446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8x |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.003 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | 0.134 |
| Number of reflections | 33445 | |
| Redundancy | 5.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10% PEG20000, 0.1M MES, 0.2M calcium acetate, 5mM zinc acetate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
