2ZJ5

Archaeal DNA helicase Hjm complexed with ADP in form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-11-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	117.205, 83.535, 96.038
Unit cell angles	90.00, 121.22, 90.00

Refinement procedure

Resolution	43.980 - 2.400
R-factor	0.216
Rwork	0.216
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zj2
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	0.384
Number of reflections	30860
<I/σ(I)>	15.4
Completeness [%]	99.2	99.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1.6M ammonium sulfate, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K