2ZIK

Crystal Structure of Human Lysozyme from Pichia pastoris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2002-02-25
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.000, 61.800, 34.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 1.810
R-factor	0.18407
Rwork	0.181
R-free	0.23759
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i1z
RMSD bond length	0.015
RMSD bond angle	1.541
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.890
High resolution limit [Å]	1.800
Rmerge	0.095
Number of reflections	11117
Completeness [%]	89.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	2.5M sodium chloride, 0.03M phosphate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K