2ZII
Crystal Structure of Yeast Vps74-N-term Truncation Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.081, 104.081, 292.759 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.100 - 3.050 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zih |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.663 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 6.570 | 3.050 |
| Rmerge | 0.142 | 0.075 | 0.529 |
| Number of reflections | 35872 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 99.8 | 98.3 | 100 |
| Redundancy | 6.1 | 5.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15% PEG 3350, 5% glycerol, 10mM citrate, 0.75mM KCl, 50mM EGTA, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
