2ZII

Crystal Structure of Yeast Vps74-N-term Truncation Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.91790
Spacegroup name	P 31 2 1
Unit cell lengths	104.081, 104.081, 292.759
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.100 - 3.050
R-factor	0.223
Rwork	0.220
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zih
RMSD bond length	0.014
RMSD bond angle	1.663
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.160
High resolution limit [Å]	3.050	6.570	3.050
Rmerge	0.142	0.075	0.529
Number of reflections	35872
<I/σ(I)>	5.6
Completeness [%]	99.8	98.3	100
Redundancy	6.1	5.4	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	15% PEG 3350, 5% glycerol, 10mM citrate, 0.75mM KCl, 50mM EGTA, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K